Journal
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
Volume 37, Issue 1, Pages -Publisher
SPRINGER
DOI: 10.1007/s11274-020-02954-8
Keywords
Endophytes; Peperomia obtusifolia; Phomopsis; Diaporthe; Ascochyta rabiei; Chickpea
Categories
Funding
- Consejo Nacional de Investigaciones Cientificas y Tecnicas de Argentina (CONICET) [PIP 524]
- FONCyT [PICT 2015 3508, PICT 2015 3592]
- Universidad Nacional de Rosario (UNR) [BIO442]
- Agencia Santafesina de Ciencia, Tecnologia e Innovacion [SecTeI IO-2017-00236]
- CONICET
Ask authors/readers for more resources
This study reported the phyto-pathogen controlling properties of 24 endophyte Phomopsis/Diaporthe isolates on the chickpea fungal pathogens, with Diaporthe infecunda and Diaporthe cf. brasiliensis showing good inhibition effects on Ascochyta rabiei.
Chickpea (Cicer arietinum L., Fabaceae) is the second most important legume after common bean (Phaseolus vulgaris L., Fabaceae) and third in production among the legumes grains worldwide. Ascochyta blight and Fusarium wilt are among the main fungal infections which cause the major losses of chickpea crop. In this work we report the phyto-pathogen controlling properties of 24 endophyte Phomopsis/Diaporthe isolates on the chickpea fungal pathogens Ascochyta rabiei, Fusarium oxysporum and Fusarium solani. The Phomopsis/Diaporthe strains were isolated amongst a total of 62 endophytic fungi from the aerial parts of the herbaceous perennial American plant Peperomia obtusifolia (Piperaceae) along with Fusarium, Septoria, Colletotrichum, Alternaria and Roussoella genera among others. Phomopsis/Diaporthe isolates were identified as Diaporthe infecunda (12 isolates), Diaporthe sackstoni (1 isolate), Diaporthe cf. brasiliensis (4 isolates) and Phomopsis cf. tuberivora (7 isolates). All the Phomopsis/Diaporthe strains antagonized A. rabiei strain AR2 with a mean of inhibition (% I) of 86.59 1.49% in dual cultures. The metabolic characterization of the Phomopsis/Diaporthe strains showed groups in three clusters which were in agreement with the taxonomic identification. Bioautographic evaluation of organic extracts showed that those of D. cf. brasiliensis and D. infecunda were better as inhibitors. Strain Po 45 was one of the most active (cluster 1, 96.87% I), and its ethyl acetate extract inhibited A. rabiei growth in a bioautographic assay until at least 10 mu g/mm applied showing a specific chromatographic band as the responsible of the A. rabiei inhibition.Graphic abstract
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available