Journal
THROMBOSIS AND HAEMOSTASIS
Volume 121, Issue 8, Pages 1066-1078Publisher
GEORG THIEME VERLAG KG
DOI: 10.1055/a-1333-7305
Keywords
heat shock factor 1; sepsis; tissue-type plasminogen activator; coagulation; microthrombosis
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Funding
- National Natural Science Fund of China [81671895, 81871610, 81471897, 81870071]
- Natural Science Foundation of Hunan Province, China [2019JJ40393]
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The study demonstrated that HSF1 knockout mice exhibited more severe tissue damage and increased coagulation activity in sepsis, along with a lack of HSF1-induced upregulation of t-PA, leading to inhibition of coagulation activity and microthrombosis.
Sepsis is a life-threatening complication of infection closely associated with coagulation abnormalities. Heat shock factor 1 (HSF1) is an important transcription factor involved in many biological processes, but its regulatory role in blood coagulation remained unclear. We generated a sepsis model in HSF1-knockout mice to evaluate the role of HSF1 in microthrombosis and multiple organ dysfunction. Compared with septic wild-type mice, septic HSF1-knockout mice exhibited a greater degree of lung, liver, and kidney tissue damage, increased fibrin / fibrinogen deposition in the lungs and kidneys, and increased coagulation activity. RNA-seq analysis revealed that tissue-type plasminogen activator (t-PA) was upregulated in the lung tissues of septic mice, and the level of t-PA was significantly lower in HSF1-knockout mice than in wild-type mice in sepsis. The effects of HSF1 on t-PA expression were further validated in HSF1-knockout mice with sepsis and in vitro in mouse brain microvascular endothelial cells using HSF1 RNA interference or overexpression under lipopolysaccharide stimulation. Bioinformatics analysis, combined with electromobility shift and luciferase reporter assays, indicated that HSF1 directly upregulated t-PA at the transcriptional level. Our results reveal, for the first time, that HSF1 suppresses coagulation activity and microthrombosis by directly upregulating t-PA , thereby exerting protective effects against multiple organ dysfunction in sepsis.
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