Journal
TALANTA
Volume 222, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.talanta.2020.121647
Keywords
Hybrid nanoflowers; Electrochemical DNA biosensors; Anticancer drug-DNA interaction; Mitomycin C; Pencil graphite electrode
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Funding
- Scientific and Technological Research Council of Turkey (TUBITAK)
- Turkish Academy of Sciences (TUBA)
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Biocompatible hybrid nanoflowers were synthesized using amino acids and developed for electrochemical monitoring of DNA. Different NFs were characterized and their performance compared, with glycine NFs showing higher sensitivity towards DNA oxidation signals. Anticancer drug interaction with DNA was also investigated using the glycine NFs-modified electrode.
In the present study, biocompatible hybrid nanoflowers (NFs) were synthesized by amino acids (glycine, L-lysine) via a simple, rapid and cost-effective methods. NFs were characterized by using FT-IR, Raman spectroscopy, XPS, SEM and EDX techniques. Modified pencil graphite electrode (PGE) surfaces with well-defined NFs were developed to electrochemical monitoring of calf thymus double stranded DNA (ctdsDNA) using differential pulse voltammetry (DPV) for the first time. SEM, EDX, cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) methods were used to characterize the surfaces obtained after modification. In comparison to L-lysine NFs (LNFs-PGE), glycine NFs (GNFs-PGE) exhibited a higher sensitivity performance towards the oxidation of guanine moiety signals. The interaction time between anticancer drug Mitomycin C (MC) and ctdsDNA was aslo investigated with GNFs-PGE.
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