Characterization, electrochemical behavior and in vitro hemocompatibility of hydroxyapatite-bioglass-iron oxide-chitosan composite coating by electrophoretic deposition

Chitosan (CS) is commonly used in the formulation of organic-inorganic coatings, which find wide applications in the biomedical field. The composite coating of hydroxyapatite (HA), bioglass (BG) and iron oxide particles (Fe3O4) were fabricated by electrophoretic deposition (EPD) on Ti-13Nb-13Zr alloy. Cathodic deposits were produced through different suspensions (1, 3, 5 wt%) of Fe3O4 particles which were prepared by the co-precipitation method. The Co-deposition of Fe3O4 particles, HA and BG resulted in the development of bioactive coatings that were embedded in the CS matrix. The coatings displayed higher zeta potential, surface roughness and wettability behavior than Ti-13Nb-13Zr alloy. The corrosion assessment revealed higher corrosion resistance of coatings which was proved by lower corrosion current density and nobler potential. The hemocompatibility analysis indicated a hemolysis ratio < 5%, which confirms the blood compatible nature of the coatings. The MTT assay shows that the Fe3O4 incorporated coatings were non-toxic and displayed prominent cell proliferation relative to the Ti-13Nb-13Zr alloy. The results revealed that the CS-HA-BG-Fe(3)O(4 )coating can be used as a favorable material for biomedical applications.

Automated summary

The study fabricated a composite coating of CS-HA-BG-Fe3O4 with bioactivity, high corrosion resistance, and excellent cell proliferation, suitable for biomedical applications.