Journal
SMALL
Volume 17, Issue 7, Pages -Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/smll.202005749
Keywords
biological responses; cell engineering; multicompartments; polymersomes; proteinosomes
Categories
Funding
- NSFC [21871069, 21474025]
- Chinese government award for outstanding students abroad by the China Scholarship Council (CSC)
- Projekt DEAL
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The study presents an efficient way to construct a hierarchical multicompartment composed of hydrogel-filled proteinosome hybrid structure with polymersomes for substance transport. The pH-responsive behavior of the system allows for reversible swelling-deswelling and on-demand cargo release in response to enzymatic reactions, fulfilling the requirements of eukaryotic cell biomimetics.
The eukaryotic cell is a smart compartment containing an outer permeable membrane, a cytoskeleton, and functional organelles, presenting part structures for life. The integration of membrane-containing artificial organelles (=polymersomes) into a large microcompartment is a key step towards the establishment of exquisite cellular biomimetics with different membrane properties. Herein, an efficient way to construct a hierarchical multicompartment composed of a hydrogel-filled proteinosome hybrid structure with an outer homogeneous membrane, a smart cytoskeleton-like scaffold, and polymersomes is designed. Specially, this hybrid structure creates a micro-environment for pH-responsive polymersomes to execute a desired substance transport upon response to biological stimuli. Within the dynamic pH-stable skeleton of the protein hydrogels, polymersomes with loaded PEGylated insulin biomacromolecules demonstrate a pH-responsive reversible swelling-deswelling and a desirable, on-demand cargo release which is induced by the enzymatic oxidation of glucose to gluconic acid. This stimulus responsive behavior is realized by tunable on/off states through protonation of the polymersomes membrane under the enzymatic reaction of glucose oxidase, integrated in the skeleton of protein hydrogels. The integration of polymersomes-based hybrid structure into the proteinosome compartment and the stimuli-response on enzyme reactions fulfills the requirements of eukaryotic cell biomimetics in complex architectures and allows mimicking cellular transportation processes.
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