Journal
SENSORS AND MATERIALS
Volume 33, Issue 1, Pages 251-260Publisher
MYU, SCIENTIFIC PUBLISHING DIVISION
DOI: 10.18494/SAM.2021.3070
Keywords
surface plasmon resonance; immunosensor; repeated measurement
Funding
- Japan Society for the Promotion of Science (JSPS, Japan) KAKENHI [JP 16K18112, 18K13763]
- Precise Measurement Technology Promotion Foundation (PMTP-F)
- Azuma Medical & Dental Research Grant
- Ministry of Education, Culture, Sports, Science and Technology (MEXT, Japan) Special Funds for Cooperative Research Project of Research Center for Biomedical Engineering
- Grants-in-Aid for Scientific Research [18K13763] Funding Source: KAKEN
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This study developed a reusable immunosensor utilizing SPR and a pH-resistant protein for rapid and repeated immunoassays. The sensor demonstrated a detection limit of 14.2 ng/mL for a-mIgG, a dynamic range of 138-30000 ng/mL, and potential for semicontinuous measurement, suggesting its application in monitoring systems for early disease detection and prevention.
Monitoring temporal changes in antigen levels, such as disease-related markers in medical care or airborne allergens in environmental medicine, has been increasing in importance because it allows one to notice the level exceeding a threshold in real time, which helps the early detection or prevention of diseases. In this study, we have developed a reusable immunosensor that exploits surface plasmon resonance (SPR) and a pH-resistant protein, ORLA85, for rapid and repeated (semicontinuous) immunoassays. After the sensor development, a model experiment using mouse (mIgG) and anti-mouse (a-mIgG) antibodies was performed. In the experiment, the sensitivity of the developed SPR immunosensor was initially evaluated using various concentrations of a-mIgG. The limit of detection of the sensor for a-mIgG was 14.2 ng/mL (0.09 nM) and the dynamic range was 138-30000 ng/mL (0.86-187.5 nM). In 10 repeated measurements of a-mIgG, the sensor signals returned to the baseline after removing a-mIgG using an acidic solution with pH 1. The measurement time was 15 min and the coefficient of variation of the sensor outputs was 7.5%. These results indicated that the developed SPR immunosensor had potential for semicontinuous measurement, suggesting its application to monitoring systems for the early detection and prevention of diseases.
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