Journal
SENSORS AND MATERIALS
Volume 33, Issue 1, Pages 241-250Publisher
MYU, SCIENTIFIC PUBLISHING DIVISION
DOI: 10.18494/SAM.2021.3073
Keywords
microfluidics; metastasis; migration; inhibitor; porous membrane
Funding
- INOUE ENRYO Memorial Grant, Toyo University
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A photolithography-free vessel-on-a-chip (VOC) was developed to simulate tumor cell extravasation, showing directional migration of tumor cells in the presence of chemical gradients and inhibition by specific inhibitors. The device allows for studying the mechanism of transendothelial migration of tumor cells and screening anti-metastatic drug candidates.
A photolithography-free vessel-on-a-chip (VOC) to simulate tumor cell extravasation is presented. A microfluidic device integrated with two pieces of porous membranes was fabricated without using photolithography. The directional migration of MDA-MB-231 cells, a metastatic tumor cell line, was observed in the presence of a concentration gradient of fetal bovine serum (FBS). Migration assays toward CXCL12 demonstrated the directional migration of the cells in the presence of a concentration gradient of chemokines. The migration was inhibited by pre-incubating the cells with AMD3100, a known inhibitor. Transendothelial migration assays with human umbilical vein endothelial cells cultured on the porous membrane revealed that there is a delay time prior to the migration of MDA-MB-231 cells through the endothelial cell layer. The present VOC will be utilized to clarify the mechanism of transendothelial migration of tumor cells as well as to screen antimetastatic drug candidates.
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