Journal
SENSORS AND ACTUATORS B-CHEMICAL
Volume 327, Issue -, Pages -Publisher
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2020.128894
Keywords
Fungi sensor; Aspergillus niger spore; Peptide ligand; Gold nanoparticle; Colorimetric detection
Funding
- National Research Foundation of Korea (NRF) - Ministry of Science, ICT and Future Planning [NRF-2016M3A7B4909581]
- Korean government (MIST) [2019R1A2C1006856]
- Basic Science Research Program through the National Research Foundation of Korea (NRF) - Ministry of Education [2017R1A6A1A03015642]
- National Research Foundation of Korea [2019R1A2C1006856] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
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The study presents a simple and rapid colorimetric strategy for detecting Aspergillus niger based on interactions between fungal spores and gold nanoparticles modified with a specific binding peptide. This approach showed high sensitivity and rapid detection time, making it suitable for the detection of large microorganisms using the specificity of ligands and the optical properties of gold nanoparticles.
The rapid and sensitive detection of allergenic fungal spores is of great interest owing to the potentially negative impact of these spores on personal and public health and safety. We present a simple and prompt colorimetric strategy for detecting Aspergillus niger based on interactions between fungal spores and gold nanoparticles (AuNPs) modified with a specific binding peptide. The A. niger spore-binding peptide ligand was identified by phage display screening, which exhibited enhanced affinity through multivalent effects with specificity over common airborne bacteria. Immobilization of peptide ligands on AuNPs enabled rapid binding to A. niger spores, resulting in a visible change in the color intensity of the supernatant after sedimentation of the spores. This simple colorimetric approach displayed a high sensitivity of similar to 50 spores and a rapid detection time of <10 min when used with a smartphone-based image analysis application. This strategy can be adapted for the simple and rapid detection of target microorganisms of relatively large size by exploiting the specificity of ligands and the optical properties of gold nanoparticles.
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