Journal
SCIENCE OF THE TOTAL ENVIRONMENT
Volume 746, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.scitotenv.2020.141089
Keywords
Methanotroph; Advanced nitrogen removal; Nitrate assimilation; Nitrogen recovery
Categories
Funding
- National Natural Science Foundation of China [51808108]
- Natural Science Foundation of Jiangsu Province [BK20180409]
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Methane is a greenhouse gas that can be released from sludge anaerobic fermentation in wastewater treatment plants. Methane is also an alternative additional carbon source for deep nitrate removal of secondary effluent. A sequencing experiment was conducted to study the efficacy of nitrate removal with methane as the sole carbon source. The maximum nitrate removal rate was 17.2 mg-N.L-1.d(-1). Nitrate removal was confirmed to arise via two pathways: aerobic methane oxidation coupled to denitrification (AME-D) contributed to 55% of the nitrate removal with the rest stemming from assimilation by methanotrophs. Additional study revealed that nitrate assimilated by methanotrophs was used for the synthesis of proteins, resulting in a protein content of 52.2% dry weight. Metagenomic sequencing revealed a high abundance of nitrate assimilation and glutamine synthetase genes, which were primarily provided by methanotrophs (mainly Methylomonas). Assimilatory nitrate removal by methanotrophs has a high potential for advanced nitrogen removal and for alleviating methane emissions. The nitrogen-rich biomass produced by nitrate absorption could also be used as a biofertilizer for nitrogen recycling. (C) 2020 Elsevier B.V. All rights reserved.
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