Journal
SCIENCE OF THE TOTAL ENVIRONMENT
Volume 748, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.scitotenv.2020.142169
Keywords
TNBP; Eisenia fetida; Cerebral ganglions; Neurotoxicity; Ca2+ overload; Osmotic imbalance
Categories
Funding
- National Natural Science Foundation of China [41773115, 41571468]
- Science and Technology Support Program of Jiangsu Province [BE2016736]
- Nanjing University Innovation Programfor PhDcandidate [CXCY19-61]
Ask authors/readers for more resources
Tri-n-butyl phosphate (TNBP) is mass-produced and widely utilized in many products, which has increasingly drawn concern about its potential environmental risks. However, little is known about the toxic mechanism on soil-dwelling organisms caused by TNBP. In this study, earthworms (Eisenia fetida) were exposed to environmentally relevant or higher concentrations of TNBP (0, 0.1, 1, and 10 mg/kg) in artificial soil for 14 days. Our results showed that TNBP accumulated in earthworm nervous tissue (cerebral ganglions). In addition, the content of glutamate in cerebral ganglions decreased compared to the control (p < 0.05). The concentration of Ca2+ in earthwormcerebral ganglions increased. However, both Na+/K+-ATPase and Ca2+-ATPase activitieswere significantly reduced compared to the control (p < 0.05), which led to neurotoxicity in earthwormnervous tissue. Furthermore, the transcriptome and metabolomics revealed the toxic mechanism in earthworm nervous tissue caused by TNBP. Results indicated that the main neurotoxicity mechanisms induced by TNBP were an osmotic imbalance and Ca2+ overload in cerebral ganglions. Our findings fill a gap in the literature on neurotoxicity mechanisms of earthwormresponse to TNBP exposure and contribute to a better understanding of the adverse effects of TNBP on soil-dwelling organisms in terrestrial ecological systems. (c) 2020 Elsevier B.V. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available