Clock genes and environmental cues coordinate Anopheles pheromone synthesis, swarming, and mating

Anopheles mating is initiated by the swarming of males at dusk followed by females flying into the swarm. Here, we show that mosquito swarming and mating are coordinately guided by clock genes, light, and temperature. Transcriptome analysis shows up-regulation of the clock genes period (per) and timeless (tim) in the head of field-caught swarming Anopheles coluzzii males. Knockdown of per and tim expression affects Anopheles gambiae s.s. and Anopheles stephensi male mating in the laboratory, and it reduces male An. coluzzii swarming and mating under semifield conditions. Light and temperature affect mosquito mating, possibly by modulating per and/or tim expression. Moreover, the desaturase gene desat1 is up-regulated and rhythmically expressed in the heads of swarming males and regulates the production of cuticular hydrocarbons, including heptacosane, which stimulates mating activity.

Automated summary

The swarming and mating behavior of Anopheles mosquitoes is regulated by clock genes, light, and temperature. The up-regulation of clock genes period and timeless affects mating behavior, while the desaturase gene desat1 plays a role in regulating the production of cuticular hydrocarbons that stimulate mating activity. Light and temperature may also influence mosquito mating behavior through modulating gene expression.