Journal
RESEARCH IN VETERINARY SCIENCE
Volume 134, Issue -, Pages 137-146Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.rvsc.2020.12.003
Keywords
Chimeric mouse; NSGS; NSG-SGM3; PDX model; HLH; Macrophage activation syndrome; Humanized model; Inflammasome
Categories
Funding
- GBM Translational Center of Excellence at the Abramson Cancer Center
Ask authors/readers for more resources
Humanized NSGS mice generated from the transplantation of CD34+ hHSC developed a fatal MAS-like phenotype characterized by hepatosplenomegaly, thrombocytopenia, anemia, and disseminated histiocytosis with infiltrates of activated macrophages. The mice exhibited a mixture of human and mouse macrophages in the affected tissues, with inflammasome activation observed in both species. The condition often developed despite low chimerism in peripheral blood, urging caution in long-term studies utilizing huNSGS mice.
Humanized immunodeficient mice generated via the transplantation of CD34+ human hematopoietic stem cells (hHSC) are an important preclinical model system. The triple transgenic NOD.Cg-Prkdc(scid)Il2rg(tm1Wjl) Tg (CMV-IL3,CSF2,KITLG)1Eav/MloySzJ (NSGS) mouse line is increasingly used as recipient for CD34+ hHSC engraftment. NSGS mice combine the features of the highly immunodeficient NSG mice with transgenic expression of the human myeloid stimulatory cytokines GM-CSF, IL-3, and Kit ligand. While generating humanized NSGS (huNSGS) mice from two independent cohorts, we encountered a fatal macrophage activation syndrome (MAS)-like phenotype resulting from the transplantation of CD34+ hHSC. huNSGS mice exhibiting this phenotype declined clinically starting at approximately 10 weeks following CD34+ hHSC engraftment, with all mice requiring euthanasia by 16 weeks. Gross changes comprised small, irregular liver, splenomegaly, cardiomegaly, and generalized pallor. Hematological abnormalities included severe thrombocytopenia and anemia. Pathologically, huNSGS spontaneously developed a disseminated histiocytosis with infiltrates of activated macrophages and hemophagocytosis predominantly affecting the liver, spleen, bone marrow, and pancreas. The infiltrates were chimeric with a mixture of human and mouse macrophages. Immunohistochemistry suggested activation of the inflammasome in both human and murine macrophages. Active Epstein-Barr virus infection was not a feature. Although the affected mice exhibited robust chimerism of the spleen and bone marrow, the phenotype often developed in the face of low chimerism of the peripheral blood. Given the high penetrance and early lethality associated with the MAS-like phenotype here described, we urge caution when considering the use of huNSGS mice for the development of long-term studies.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available