Journal
PROTEOMICS
Volume 21, Issue 6, Pages -Publisher
WILEY
DOI: 10.1002/pmic.202000305
Keywords
malaria; mass spectrometry; Plasmodium berghei; proteome; sporozoites; timsTOF PRO
Funding
- Laboratoire d'Excellence ParaFrap [ANR-11-LABX-0024]
- Agence Nationale de la Recherche [ANR-16-CE15-0004, ANR-16-CE15-0010]
- Fondation pour la Recherche Medicale [EQU201903007823]
- Conseil Regional d'Ile-de-France
- National Institute for Health and Medical Research (INSERM)
- Biology, Health and Agronomy Infrastructure (IBiSA)
- Sorbonne Universite
- Agence Nationale de la Recherche (ANR) [ANR-16-CE15-0004, ANR-16-CE15-0010] Funding Source: Agence Nationale de la Recherche (ANR)
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The study identified the proteome of P. berghei sporozoites using a highly sensitive mass spectrometer, providing a reference proteome for further exploration of malaria biology. This study highlights the unprecedented sensitivity of the timsTOF PRO system for deep proteomic analysis from limited sample amounts.
Sporozoites of the malaria parasite Plasmodium are transmitted by mosquitoes and infect the liver for an initial and obligatory round of replication, before exponential multiplication in the blood and onset of the disease. Sporozoites and liver stages provide attractive targets for malaria vaccines and prophylactic drugs. In this context, defining the parasite proteome is important to explore the parasite biology and to identify potential targets for antimalarial strategies. Previous studies have determined the total proteome of sporozoites from the two main human malaria parasites, P. falciparum and P. vivax, as well as P. yoelii, which infects rodents. Another murine malaria parasite, P. berghei, is widely used to investigate the parasite biology. However, a deep view of the proteome of P. berghei sporozoites is still missing. To fill this gap, we took advantage of the highly sensitive timsTOF PRO mass spectrometer, combined with three alternative methods for sporozoite purification, to identify the proteome of P. berghei sporozoites using low numbers of parasites. This study provides a reference proteome for P. berghei sporozoites, identifying a core set of proteins expressed across species, and illustrates how the unprecedented sensitivity of the timsTOF PRO system enables deep proteomic analysis from limited sample amounts.
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