Journal
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
Volume 118, Issue 1, Pages -Publisher
NATL ACAD SCIENCES
DOI: 10.1073/pnas.2017120118
Keywords
cigarette smoke; rheumatoid arthritis; Th17; exosomes; osteoclastogenesis
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Funding
- Sao Paulo Research Foundation (FAPESP) [2012/02438-0, 2013/08216-2]
- University of Sao Paulo (NAP-DIN) [11.1.21625.01.0]
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Transcriptomic analysis revealed that microRNA-132 is induced specifically in Th17 cells in the presence of AhR agonist or CS-enriched medium. This induced miRNA132 acts as a proinflammatory mediator by promoting osteoclastogenesis through down-regulating COX2, exacerbating the development of RA.
Rheumatoid arthritis (RA) is a chronic inflammatory disease characterized by joint destruction and severe morbidity. Cigarette smoking (CS) can exacerbate the incidence and severity of RA. Although Th17 cells and the Aryl hydrocarbon receptor (AhR) have been implicated, the mechanism by which CS induces RA development remains unclear. Here, using transcriptomic analysis, we show that microRNA-132 is specifically induced in Th17 cells in the presence of either AhR agonist or CS-enriched medium. miRNA132 thus induced is packaged into extracellular vesicles produced by Th17 and acts as a proinflammatory mediator increasing osteoclastogenesis through the down-regulation of COX2. In vivo, articular knockdown of miR-132 in murine arthritis models reduces the number of osteoclasts in the joints. Clinically, RA patients express higher levels of miR-132 than do healthy individuals. This increase is further elevated by cigarette smoking. Together, these results reveal a hitherto unrecognized mechanism by which CS could exacerbate RA and further advance understanding of the impact of environmental factors on the pathogenesis of chronic inflammatory diseases.
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