An investigation into influence of acetylated cellulose nanofibers on properties of PCL/Gelatin electrospun nanofibrous scaffold for soft tissue engineering

In this study, the effective role of incorporation of cellulose nanofibers (CNF) and modified acetylated cellulose nanofibers (ACNF) on mechanical, physical, and biological properties of poly (c-caprolactone) (PCL)/gelatin (Gel) electrospun nanofibrous scaffold was individually investigated. It was noticed that mechanical and biological properties of the scaffolds were considerably affected by the filler type and content. Although, by addition of 2 wt% ACNF, the ultimate tensile strength (UTS) of the PCL/Gel was remarkably enhanced from 2.5 +/- 0.1 MPa to 4.3 +/- 0.1 MPa due to homogeneous dispersion of the ACNF, however, the degradation rate of PCL/Gel scaffold was reduced about 1.66 times. Moreover, the studies on the interactions between hybrid scaffolds and fibroblast cells revealed that the incorporation of ACNF into scaffold not only showed no cytotoxic, but also promoted cell proliferation. In conclusion, PCL/Gel nanocomposite scaffolds reinforced by ACNFs show an excellent potential as a promising candidate for soft tissue engineering applications.

Automated summary

The study demonstrated that the addition of ACNF significantly improved the tensile strength of PCL/Gel scaffold, while reducing the degradation rate. Incorporation of ACNF showed no cytotoxic effects and promoted cell proliferation.