Journal
PLANTA MEDICA
Volume 87, Issue 4, Pages 283-293Publisher
GEORG THIEME VERLAG KG
DOI: 10.1055/a-1295-8606
Keywords
apoptosis; Bcl-2 family proteins; Dendrobium densiflorum; erianthridin; extracellular signal-regulated kinase; Orchidaceae; non-small cell lung cancer cells
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Funding
- Ratchadaphiseksomphot Endowment Fund, Chulalongkorn University [CU-GR_62_57_33_04]
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Erianthridin, a compound isolated from Dendrobium formosum, has shown promising cytotoxic effects in non-small cell lung cancer cells by inducing apoptosis. Mechanistic studies indicate that erianthridin can modulate apoptosis-related protein markers and mediate apoptosis through the regulation of Bcl-2 family proteins. In vitro assays demonstrate the potential of erianthridin to suppress lung cancer cell proliferation.
Due to the high mortality of lung cancer, natural derivative compounds have been promoted as versatile sources for anticancer drug discovery. Erianthridin, a phenanthrene compound isolated from Dendrobium formosum, exhibits intriguing apoptosis-inducing effects in non-small cell lung cancer cells. Apoptotic nuclei staining assays showed that apoptotic cells with DNA fragmentation and apoptotic bodies were apparent, and an increase in annexin V-FITC-positive cells were found in cells treated with erianthridin. The apoptosis protein markers for cleaved caspase-3 and cleaved poly-ADP-ribose polymerase were significantly upregulated in response to erianthridin. A mechanistic investigation revealed that erianthridin was able to attenuate extracellular signal-regulated kinase activity and thereby mediate apoptosis through the modulation of Bcl-2 family protein levels. U0126, an extracellular signal-regulated kinase inhibitor, augmented the apoptosis-inducing effect of erianthridin; in contrast, overexpression of exogenous extracellular signal-regulated kinase substantially abrogated erianthridin activity. Furthermore, an in vitro 3D tumorigenesis assay showed that erianthridin was able to potentially suppress lung cancer cell proliferation. This study is the first to report a promising cytotoxic effect of erianthridin, which provides preclinical evidence for further research and development of this compound.
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