Journal
PLANT PHYSIOLOGY AND BIOCHEMISTRY
Volume 157, Issue -, Pages 359-369Publisher
ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER
DOI: 10.1016/j.plaphy.2020.10.035
Keywords
Rice (Oryza sativa L.); Map-based cloning; Leaf morphology; Nitrogen transport efficiency; Grain development
Categories
Funding
- National GMO New Variety Breeding Program of PRC [2016ZX08011-001]
- National Natural Science Foundation of China [31861143006, 31770195, 31901483]
- National Key Research and Development Program [2016YFD0101801]
- Jiangxi Natural Science Foundation [20202ACBL205007]
- Zhejiang Provincial Ten Thousand Talent Program Project [2018R52025, 2019R52031]
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Leaves are the primary structures responsible for photosynthesis, making leaf morphology one of the most important traits of rice plant architecture. Both plant architecture and nutrient utilization jointly affect rice yield, however, their molecular association is still poorly understood. We identified a rice mutant, leaf width 5 (lw5), that displayed small grains and wide leaves and possesses characteristics typical of a small sink and a large source. Map-based cloning and CRISPR-Cas9 gene editing indicated that LW5 affects both the plant architecture and yield. It is an allele of D1, encoding the rice G protein a subunit. The loss of LW5 functioning leads to an increase in the rate of photosynthesis, vascular bundles, and chlorophyll content. However, the grain-straw ratio and the rate of grain filling decreased significantly. The detection results of 15 N-ammonium nitrate and an expression analysis of genes associated with nitrogen demonstrated that LW5 serves an important role in nitrate uptake and transport. LW5 affects plant architecture and grain size by regulating nitrogen transfer. These results provide a theoretical foundation for further research surrounding the molecular mechanism of source-sink balance in rice and suggest novel methods of molecular design for the cultivation of breeding super rice in ideal plant types.
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