Journal
PLANT PHYSIOLOGY AND BIOCHEMISTRY
Volume 158, Issue -, Pages 308-320Publisher
ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER
DOI: 10.1016/j.plaphy.2020.11.016
Keywords
Avocado; AP2/ERF transcription Factor; Gene expression; Fatty acid accumulation
Categories
Funding
- National Natural Science Foundation of China [31701883]
- Natural Science Foundation of Hainan Province of China [2019RC264]
- Integrated demonstration of key techniques for the industrial development of featured crops in rocky desertification areas of Yunnan-Guangxi -Guizhou provinces [SMH 20192021]
- Ministry of agriculture and rural affairs hot technology pilot demonstration project [20203578]
- Ministry of agriculture and rural affairs agricultural variety improvement special funds [20201982]
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The study systematically analyzed AP2/ERF family members in avocado and identified 10 highly expressed PaAP2/ERF genes potentially related to fatty acid accumulation in avocado fruit. This analysis provides a foundation for future studies on the functions of AP2/ERF associated with fatty acid accumulation.
Fatty acids in avocado fruit are crucial components influencing taste as well as fruit quality and nutritional value. Changes to fatty acid contents and concentrations in avocado fruit are important because of the associated effects on sensory properties. Hence, plant physiologists and molecular biologists interested in elucidating the influence of transcription factors on fatty acid accumulation in avocado fruit. In this study, APETALA2/ethyleneresponsive factor (AP2/ERF) family members in avocado (Persea americana Mill.) were systematically and comprehensively analyze to identify potential PaAP2/ERF genes related to fatty acid accumulation. The results of bioinformatics analysis and the expression profiles of the AP2/ERF members suggested that 10 highly expressed PaAP2/ERF genes may encode transcription factors with functions related to the fatty acid accumulation in the avocado mesocarp. Furthermore, PaWRI1 and PaWRI2, two AP2/ERF transcription factor genes in avocado, were functionally characterized regarding their effects on fatty acid accumulation. The transcriptome and biochemical analyses of PaWRI1-2-overexpressing transgenic tomato plants revealed the up-regulated expression of 17 unigenes related to fatty acid synthesis and triacylglycerol assembly as well as increased fatty acid contents relative to the corresponding levels in the wild-type plants. In contrast, the overexpression of PaWRI2 in transgenic tomato plants up-regulated the expression of only six unigenes associated with fatty acid synthesis and triacylglyceml assembly and negligibly affected fatty acid accumulation when compared with wild-type plants. This systematic analysis provides a foundation for future studies regarding AP2/ERF functions associated with fatty acid accumulation.
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