Journal
PLANT JOURNAL
Volume 105, Issue 1, Pages 136-150Publisher
WILEY
DOI: 10.1111/tpj.15046
Keywords
cell wall acylation; p‐ coumaric acid; hydroxycinnamic acids; grass xylan; lignin; lignocellulose; saccharification
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Funding
- Coordination of Enhancement of Higher Education Personnel (CAPES)
- Brazilian National Council for Scientific and Technological Development [CNPq - GM/GD - 141076/2016-0]
- EMBRAPA Macroprogram SEG [02.12.01.008.00.00]
- BBSRC [BBS/E/C/000I0250, BB/K013335/1, BB/K007599/1] Funding Source: UKRI
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The study identified a BAHD gene involved in p-coumaroylation of AX in the cell wall of Setaria viridis. Silenced lines with reduced levels of SvBAHD05 showed decreased ester-linked p-coumarate and improved biomass saccharification. This indicates the potential of targeting SvBAHD05 for enhancing biomass digestibility in biofuels, biorefineries, and animal feed.
Grass cell walls have hydroxycinnamic acids attached to arabinosyl residues of arabinoxylan (AX), and certain BAHD acyltransferases are involved in their addition. In this study, we characterized one of these BAHD genes in the cell wall of the model grass Setaria viridis. RNAi silenced lines of S. viridis (SvBAHD05) presented a decrease of up to 42% of ester-linked p-coumarate (pCA) and 50% of pCA-arabinofuranosyl, across three generations. Biomass from SvBAHD05 silenced plants exhibited up to 32% increase in biomass saccharification after acid pre-treatment, with no change in total lignin. Molecular dynamics simulations suggested that SvBAHD05 is a p-coumaroyl coenzyme A transferase (PAT) mainly involved in the addition of pCA to the arabinofuranosyl residues of AX in Setaria. Thus, our results provide evidence of p-coumaroylation of AX promoted by SvBAHD05 acyltransferase in the cell wall of the model grass S. viridis. Furthermore, SvBAHD05 is a promising biotechnological target to engineer crops for improved biomass digestibility for biofuels, biorefineries and animal feeding.
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