Journal
PHYTOTHERAPY RESEARCH
Volume 35, Issue 5, Pages 2568-2578Publisher
WILEY
DOI: 10.1002/ptr.6987
Keywords
antiinflammatory activity; Physalis alkekengi L; var; franchetii; RNA‐ sequencing; withanolide
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Funding
- Natural Science Foundation of Fujian Province [2019J05104]
- Quanzhou Science and technology plan project [2018Z030]
- Project of Key Laboratory of Preparation and Functional Development of Algae Active Substances in Fujian Province [2017FZSK03]
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The fruits of Physalis alkekengi L. var. franchetii in China contain various compounds, among which Physapubescin M shows significant anti-inflammatory effects on macrophages, reducing the expression of inflammatory proteins and cytokines in a dose-dependent manner. Transcriptomic analyses reveal the potential mechanism of withanolides in inhibiting inflammation, providing a theoretical basis for the use of Physalis fruits.
In China, the fruits of Physalis alkekengi L. var. franchetii, which are conventionally utilized as edible berry, have attracted wide attention due to its significant biological activities. In the present study, phytochemical studies on the fruits of Physalis plants afforded six compounds, including two new withanolides (1-2) and four known agnologues (3-6). The inhibitory effects of these compounds on the formation of nitric oxide (NO) stimulated by lipopolysaccharide (LPS) in RAW264.7 macrophages were evaluated. Physapubescin M (1), with IC50 value of 1.58 mu M, was selected for further study. The protein expression of COX-2 and iNOS, and LPS-induced production of cytokines (IL-6, IL-1 beta and TNF-alpha) were reduced by physapubescin M (1) in a dose-dependent way. In addition, transcriptomic analyses were conducted to profile gene expression alterations in LPS-induced RAW264.7 cells upon treatment of physapubescin M (1) and the potential antiinflammatory mechanism of withnolides was mentioned. These results provide broad view to the underlying antiinflammatory mechanism of withnolides, and give a theoretical basis for the utilization of the fruits of P. alkekengi L. var. franchetii.
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