Journal
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
Volume 173, Issue -, Pages -Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.pestbp.2021.104777
Keywords
Antifungal; Fumigation; Membrane; Reactive oxygen species; Mitochondrial dysfunction
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Funding
- National Natural Science Foundation of China [31101502]
- Qing Lan Project of Jiangsu Province
- China Agriculture Research System [CARS-10-B03]
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This study elucidated that the volatile organic compounds released by Pseudomonas chlororaphis subsp. aureofaciens SPS-41 exerted antifungal activity against Ceratocystis fimbriata by inducing oxidative stress and mitochondrial dysfunction in the fungal cells.
Ceratocystis fimbriata is the pathogen of black rot disease, which widely exists in sweet potato producing areas all over the world. The antifungal activity of volatile organic compounds (VOCs) released by Pseudomonas chlororaphis subsp. aureofaciens SPS-41 against C. fimbriata was reported in our previous study. In this study, we attempted to reveal the underlying antifungal mechanism of SPS-41 volatiles. Our results showed that the VOCs released by SPS-41 caused the morphological change of hyphae, destroyed the integrity of cell membrane, reduced the content of ergosterol, and induced massive accumulation of reactive oxygen species in C. fimbriata cells. Furthermore, SPS-41 fumigation decreased the mitochondrial membrane potential, acetyl-CoA and pyruvate content of C. fimbriata cells, as well as the mitochondrial dehydrogenases activity. In addition, the VOCs generated by SPS-41 reduced the intracellular ATP content and increased the extracellular ATP content of C. fimbriata. In summary, SPS-41 fumigation exerted its antifungal activity by inducing oxidative stress and mitochondrial dysfunction in C. fimbriata.
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