Strategies for enhancing the efficiency of RNA interference in insects

Low RNA interference (RNAi) efficiency in many insect pests has significantly prevented its widespread application for insect pest management. This article provides a comprehensive review of recent research in developing various strategies for enhancing RNAi efficiency. Our review focuses on the strategies in target gene selection and double-stranded RNA (dsRNA) delivery technologies. For target gene selection, genome-wide or large-scale screening strategies have been used to identify most susceptible target genes for RNAi. Other strategies include the design of dsRNA constructs and manipulate the structure of dsRNA to maximize the RNA efficiency for a target gene. For dsRNA delivery strategies, much recent research has focused on the applications of complexed or encapsulated dsRNA using various reagents, polymers, or peptides to enhance dsRNA stability and cellular uptake. Other dsRNA delivery strategies include genetic engineering of microbes (e.g. fungi, bacteria, and viruses) and plants to produce insect-specific dsRNA. The ingestion of the dsRNA-producing organisms or tissues will have lethal or detrimental effects on the target insect pests. This article also identifies obstacles to further developing RNAi for insect pest management and suggests future avenues of research that will maximize the potential for using RNAi for insect pest management. (c) 2021 Society of Chemical Industry

Automated summary

The article provides a comprehensive review of strategies for enhancing RNAi efficiency in insect pests, focusing on target gene selection and dsRNA delivery technologies. Recent research has focused on genome-wide screening for target gene selection and designing dsRNA constructs for maximal efficiency. In terms of dsRNA delivery, research has explored complexing or encapsulating dsRNA using various agents to enhance stability and uptake, as well as genetic engineering of microbes and plants for insect-specific dsRNA production.