Journal
PEST MANAGEMENT SCIENCE
Volume 77, Issue 4, Pages 1796-1805Publisher
JOHN WILEY & SONS LTD
DOI: 10.1002/ps.6204
Keywords
RNAi; cotton mealybug; dsRNA; in planta‐ induced transient gene silencing; Krü ppel
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Funding
- SERB-Department of Science & Technology, Government of India [EEQ/2016/000221]
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This study provides evidence for the effectiveness of using RNAi technology to target the Kruppel homologue-1 gene in a hemipteran pest, P. solenopsis. The results show significant downregulation of gene expression for Kruppel homologue-1, demonstrating the potential of RNAi technology in pest control.
BACKGROUND Cotton is a cash crop majorly affected by many hemipteran pests, among them the cotton mealybug, Phenacoccus solenopsis. Cotton mealybug attack has a devastating effect on cotton production and causes huge yield losses. RESULTS In this study, 25 potential RNA interference (RNAi) target genes were selected from the iBeetle database and a transcriptome data set for P. solenopsis. To assess the effectiveness of the selected target genes, three methods were utilized to deliver double-stranded (ds)RNA (ingestion, artificial diet bioassay and transient gene silencing). dsRNA molecules at different concentrations were fed to insects and insect mortality was recorded for each target gene. Based on the mortality data, three genes, Kruppel homologue-1, ADP-ATP/Translocase and IDGF-1, were selected for further gene expression studies using a reduced concentration of dsRNA (5 mu g/ml). Of the three genes, Kruppel homologue-1 showed significantly downregulated expression (by 70.81% and 84.33%) at two different time points (8 and 14 days). An RNAi silencing construct was designed for Kruppel homologue-1 under control of the double enhancer CamV35S promoter in the plant binary vector. Significant downregulation of gene expression, by 66.69% and 81.80%, was found for Kruppel homologue-1 using transient gene silencing at the same time intervals. CONCLUSION This work provides the first evidence for targeting the Kruppel homologue-1 gene in a hemipteran pest, P. solenopsis, using RNAi technology through oral delivery and in planta-based transient gene silencing methods.
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