4.8 Article

Identification of an α-Oxoamine Synthase and a One-Pot Two-Step Enzymatic Synthesis of α-Amino Ketones

Journal

ORGANIC LETTERS
Volume 23, Issue 1, Pages 37-41

Publisher

AMER CHEMICAL SOC
DOI: 10.1021/acs.orglett.0c03600

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Funding

  1. National Key Research and Development Program of China [2019YFC0312501]

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Alb29 is an alpha-oxoamine synthase involved in albogrisin biosynthesis in Streptomyces albogriseolus MGR072, responsible for incorporating L-glutamate into acyl-coenzyme A substrates. A one-pot enzymatic system was established to synthesize seven alpha-amino ketones by combining Alb29 with Mgr36 (an acyl-coenzyme A ligase). When these alpha-amino ketones were fed into the alb29 knockout strain Delta alb29, albogrisin analogs with different side chains were observed.
Alb29, an alpha-oxoamine synthase involved in albogrisin biosynthesis in Streptomyces albogriseolus MGR072, was characterized and responsible for the incorporation of L-glutamate to acyl-coenzyme A substrates. Combined with Alb29 and Mgr36 (an acyl-coenzyme A ligase), a one-pot enzymatic system was established to synthesize seven a-amino ketones. When these alpha-amino ketones were fed into the alb29 knockout strain Delta alb29, respectively, the albogrisin analogs with different side chains were observed.

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