Journal
NUCLEOSIDES NUCLEOTIDES & NUCLEIC ACIDS
Volume 40, Issue 2, Pages 194-211Publisher
TAYLOR & FRANCIS INC
DOI: 10.1080/15257770.2020.1859116
Keywords
MicroRNA; let-7; LIN28B; RNA structure; SHAPE
Categories
Funding
- CONACyT [A1-S-9160, 300073, BFU2017-84492-R]
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The study revealed the importance of the Let-7:LIN28 regulatory loop in miRNA regulation, investigated the binding differences between LIN28B and pre-let-7 members, and uncovered diverse structural patterns within the pre-let-7 family through SHAPE mapping.
The Let-7:LIN28 regulatory loop is a paradigm in miRNA regulation. LIN28 harbors two RNA binding domains, which interact with well-conserved sequences in pre-let-7 RNAs, the GNGAY and the GGAG motifs. Here, the differential binding between LIN28B and pre-let-7 members was associated with the structural characteristics of the pre-let-7 family mapped by SHAPE, uncovering diverse structural patterns within pre-let-7 members. Pre-let-7 mutants supported a relevant role of the GGAG motif location and the preE-stem stability for the interaction with LIN28B. Based on these results, we propose a core RNA structure for LIN28B interaction.
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