ProThermDB: thermodynamic database for proteins and mutants revisited after 15 years

ProThermDB is an updated version of the thermodynamic database for proteins and mutants (ProTherm), which has similar to 31 500 data on protein stability, an increase of 84% from the previous version. It contains several thermodynamic parameters such as melting temperature, free energy obtained with thermal and denaturant denaturation, enthalpy change and heat capacity change along with experimental methods and conditions, sequence, structure and literature information. Besides, the current version of the database includes about 120 000 thermodynamic data obtained for different organisms and cell lines, which are determined by recent high throughput proteomics techniques using whole-cell approaches. In addition, we provided a graphical interface for visualization of mutations at sequence and structure levels. ProThermDB is crosslinked with other relevant databases, PDB, UniProt, PubMed etc. It is freely available at https://web.iitm.ac.in/bioinfo2/prothermdb/index.html without any login requirements. It is implemented in Python, HTML and JavaScript, and supports the latest versions of major browsers, such as Firefox, Chrome and Safari.

Automated summary

ProThermDB is an updated version of a thermodynamic database for proteins and mutants, containing a vast amount of data on protein stability and providing a graphical interface for mutation visualization. It also includes a large amount of thermodynamic data from different organisms and cell lines, obtained using recent high throughput techniques.