Journal
NUCLEIC ACIDS RESEARCH
Volume 49, Issue 2, Pages -Publisher
OXFORD UNIV PRESS
DOI: 10.1093/nar/gkaa1227
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Funding
- French Ministry
- INSA Lyon grants [BQR 2016]
- IXXI
- AgenceNationale de laRecherche [ANR-18-CE45-0006-01]
- Breakthrough Phytobiome IDEX LYON project, Universite de Lyon Programme d'investissements d'Avenir [ANR16-IDEX-0005]
- Centre National de la Recherche Scientifique
- Universite de Lyon
- CNRS recurrent funding
- INSA grant
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The study reveals the importance of IHF heterodimeric protein in bacterial adaptation and pathogenicity, as its absence affects the transcriptional organization and virulence gene expression of the bacteria.
Bacterial pathogenic growth requires a swift coordination of pathogenicity function with various kinds of environmental stress encountered in the course of host infection. Among the factors critical for bacterial adaptation are changes of DNA topology and binding effects of nucleoid-associated proteins transducing the environmental signals to the chromosome and coordinating the global transcriptional response to stress. In this study, we use the model phytopathogen Dickeya dadantii to analyse the organisation of transcription by the nucleoid-associated heterodimeric protein IHF. We inactivated the IHF alpha subunit of IHF thus precluding the IHF alpha beta heterodimer formation and determined both phenotypic effects of ihfA mutation on D. dadantii virulence and the transcriptional response under various conditions of growth. We show that ihfA mutation reorganises the genomic expression by modulating the distribution of chromosomal DNA supercoils at different length scales, thus affecting many virulence genes involved in both symptomatic and asymptomatic phases of infection, including those required for pectin catabolism. Altogether, we propose that IHF heterodimer is a 'transcriptional domainin' protein, the lack of which impairs the spatiotemporal organisation of transcriptional stress-response domains harbouring various virulence traits, thus abrogating the pathogenicity of D. dadantii.
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