Journal
NUCLEIC ACIDS RESEARCH
Volume 48, Issue 22, Pages 12648-12659Publisher
OXFORD UNIV PRESS
DOI: 10.1093/nar/gkaa1050
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Funding
- Japan Science and Technology Agency (JST) [PRESTO program]
- Japan Society for the Promotion of Science (JSPS) [20H05785, 15H01345, 17H01813, 18K19834, 20H00619, 16H05089, 20H03388, 20K21406]
- Takeda Science Foundation
- Japan Society for the Promotion of Science [20H03388]
- RIKEN ['Epigenome Manipulation Project' of the All-RIKEN Projects]
- Grants-in-Aid for Scientific Research [20H00619, 20H05785, 20H03388, 20K21406, 17H01813, 16H05089, 15H01345, 18K19834] Funding Source: KAKEN
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Eukaryotic transcription is epigenetically regulated by chromatin structure and post-translational modifications (PTMs). For example, lysine acetylation in histone H4 is correlated with activation of RNA poly- merase I- II- and III-driven transcription from chro- matin templates, which requires prior chromatin remodeling. However, quantitative understanding of the contribution of particular PTM states to the sequential steps of eukaryotic transcription has been hampered partially because reconstitution of a chromatin template with designed PTMs is difficult. In this study, we reconstituted a di-nucleosome with site-specifically acetylated or unmodified histone H4, which contained two copies of the Xenopus somatic 5S rRNA gene with addition of a unique sequence detectable by hybridization-assisted fluorescence correlation spectroscopy. Using a Xenopus oocyte nuclear extract, we analyzed the time course of accumulation of nascent 5S rRNA-derived transcripts generated on chromatin templates in vitro. Our mathematically described kinetic model and fitting analysis revealed that tetra-acetylation of histone H4 at K5/K8/K12/K16 increases the rate of transcriptionally competent chromatin formation similar to 3-fold in comparison with the absence of acetylation. We provide a kinetic model for quantitative evaluation of the contribution of epigenetic modifications to chromatin transcription.
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