Journal
NEUROSCIENCE
Volume 458, Issue -, Pages 87-98Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.neuroscience.2021.01.024
Keywords
Ca2+/calmodulin-dependent protein kinase (CaMK); cAMP responsive element-binding protein (CREB); dendrite; cerebellum; small interfering RNA (siRNA); single-cell electroporation
Categories
Funding
- Japan Society for the Promotion of Science [KAKENHI 22500312, 25430040]
- Nagoya City University
- Grants-in-Aid for Scientific Research [25430040] Funding Source: KAKEN
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The study showed that CaMKII alpha, II beta, and IV play a crucial role in the dendritic differentiation of Purkinje cells, and inhibiting all three CaMKs simultaneously significantly affects dendritic branching development and phosphorylation of CREB in Purkinje cells.
Cerebellar Purkinje cells develop the most elaborate dendritic trees among neurons in the brain. To examine the role of Ca2+/calmodulin-dependent protein kinase (CaMK) II alpha, II beta and IV in the dendritic differentiation of Purkinje cells, we introduced siRNA against these CaMKs into Purkinje cells in cerebellar cell cultures using a single-cell electroporation technique. Single-cell electroporation enables us to transfer siRNA into specific cells within a heterogeneous cell population. In addition, we can easily and reliably transfer multiple types of siRNA into a cell simply by loading them together in one micropipette. Any one of the siRNA against CaMKII alpha, II beta and IV (single knockdown) or any combinations of two of the siRNA against these CaMKs (double knockdown) had no significant effects on the dendritic differentiation of Purkinje cells. However, the combination of all three siRNA against these CaMKs (triple knockdown) inhibited the branching of Purkinje cell dendrites. Furthermore, the triple knockdown reduced the phosphorylation of CREB in Purkinje cells. These findings suggest the promotion of dendritic differentiation of Purkinje cells by CaMKII alpha, II beta and IV and the possible involvement of phosphorylation of CREB as a common substrate of these CaMKs. (C) 2021 IBRO. Published by Elsevier Ltd. All rights reserved.
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