CRISPR technologies for precise epigenome editing

Qi and colleagues review CRISPR-based epigenome engineering technologies to modulate histone and DNA modifications and to perturb DNA and RNA regulatory elements and chromatin organization. The epigenome involves a complex set of cellular processes governing genomic activity. Dissecting this complexity necessitates the development of tools capable of specifically manipulating these processes. The repurposing of prokaryotic CRISPR systems has allowed for the development of diverse technologies for epigenome engineering. Here, we review the state of currently achievable epigenetic manipulations along with corresponding applications. With future optimization, CRISPR-based epigenomic editing stands as a set of powerful tools for understanding and controlling biological function.

Automated summary

The review discusses CRISPR-based epigenome engineering technologies for modulating histone and DNA modifications, perturbing DNA and RNA regulatory elements, and chromatin organization. The epigenome involves complex cellular processes governing genomic activity. Repurposing prokaryotic CRISPR systems has enabled the development of diverse technologies in epigenome engineering.