Journal
NATURAL PRODUCT RESEARCH
Volume 36, Issue 5, Pages 1337-1341Publisher
TAYLOR & FRANCIS LTD
DOI: 10.1080/14786419.2020.1868461
Keywords
Vitex polygama; orientin; isoorientin; 20-hydroxyecdysone; tuberculosis; anti-inflammatory
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Funding
- Coordination of Superior Level Staff Improvement (CAPES)
- National Council for Scientific and Technological Development (CNPq)
- Carlos Chagas Filho Research Support Foundation of the State of Rio de Janeiro (FAPERJ)
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The compound orientin has been found to have antimycobacterial activity against drug-resistant strains of tuberculosis, as well as immunomodulatory effects and low cytotoxicity.
Tuberculosis (TB) remains a worldwide public health threat because of the emergence of resistant strains and subsequent inappropriate response to current therapy. We have been studying the restinga plants' antimycobacterial and anti-inflammatory potential. Dichloromethane fraction (DCM) from Vitex polygama Cham. showed high activity against Mycobacterium tuberculosis (Mtb) H37Rv. In this context, DCM fraction and isolated compounds were investigated against Mtb H37Rv and M299 (MDR strain) and for their immunomodulatory and cytotoxicity actions. Orientin showed the best antimycobacterial effect against Mtb M299 MDR strain (MIC50 15.4 +/- 1.6 mu g/mL), capacity of inhibiting NO production by macrophages (IC50 6.5 +/- 1.2 mu g/mL) and no significant cytotoxicity. The antimycobacterial effect of orientin was also observed on Mtb H37Rv intracellular growth in RAW 264.7 macrophages (MIC50 3.5 +/- 1.1 and MIC90 9.1 +/- 1.0 mu g/mL). This is the first report describing the antimycobacterial effect of orientin, in both extra- and intracellular growth.
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