Isolation and identification of two new compounds from the seeds of Moringa oleifera and their antiviral and anti-inflammatory activities

Eleven compounds were isolated from methanol extract taken from Moringa oleifera seeds, including two previously unknown and nine known compounds. These compounds were authenticated as a carbamate, three phenylglycosides, four phenol glycosides, two nucleosides, and one flavonoid. Their chemical structures were elucidated using 1 D/2D nuclear magnetic resonance and high resolution-MS. Antivirus activity analyses revealed that Moringa A, glucomoringin, and Vitexin possessed strong inhibitory effects against the H1N1 virus, having IC50 values in the range of IC50 = 0.26 +/- 0.03, 0.98 +/- 0.17, and 3.42 +/- 0.37 mu g/mL, respectively. Furthermore, these three compounds could decrease the levels of TNF-alpha, IL-6, and IL-1 beta, which occur in hosts because of H1N1 infections.

Automated summary

Eleven compounds were isolated from methanol extract of Moringa oleifera seeds, including two new and nine known compounds. These compounds showed strong inhibitory effects against H1N1 virus and could reduce the levels of inflammatory cytokines in hosts infected with H1N1.