Real-Time Dissecting the Dynamics of Drug Transportation in the Live Brain

Brain diseases are becoming a more and more serious threat to human health. Many critical properties of the transport mechanisms of drugs in live brains remain poorly understood. In this work, single-particle tracking was used to dissect the transport dynamics of wheat germ agglutinin (WGA) in live brain and characterize the geometry and rheology of the extracellular space (ECS). The results revealed that the movements of WGA were influenced by the specific-binding molecules and the nature of the ECS. We further analyzed the mobility behaviors of WGA globally and quantitatively and found that movement of WGA in brain cells of acute slices was an active transport process associated with actin filaments and microtubules. This work paves the way for studies aiming at characterizing the biophysics of drug transport in the context of live brains, which may contribute to developing potential new therapeutic applications for brain diseases.

Automated summary

Brain diseases pose an increasing threat to human health, while understanding the transport mechanisms of drugs in live brains remains limited. Through tracking the dynamics of WGA in live brains, this study revealed influences of specific-binding molecules and ECS nature on transport dynamics, paving the way for exploring the biophysics of drug transport in live brains and identifying potential new therapeutic applications for brain diseases.