Journal
MOLECULES
Volume 25, Issue 22, Pages -Publisher
MDPI
DOI: 10.3390/molecules25225470
Keywords
dark chocolate; milk chocolate; cocoa shell; CBE; dairy ingredients; polyphenol; flavonoid
Funding
- Croatian Science Foundation [UIP 2017-05-8709]
- European Union from the European Regional Development Fund [KK.01.1.1.02.0005]
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Chocolate is considered as both caloric and functional food. Its nutritional properties may be improved by addition of fiber; however, this may reduce polyphenols content. The aim of this research was to determine the influence of cocoa shell addition (as a source of fiber) and its combination with different ingredients (cocoa butter equivalents (CBE), emulsifiers, dairy ingredients) on polyphenols of dark and milk chocolates. Total polyphenol (TPC) and total flavonoid (TFC) contents were determined spectrophotometrically, identification and quantification of individual compounds by high pressure liquid chromatography and antioxidant capacity by ferric reducing antioxidant power (FRAP) assay. Results showed that even though addition of cocoa shell to chocolate results in reduced contents of TPC, TFC, and individual compounds, it is not significant compared to ones reported by other authors for commercial chocolates. Other ingredients influence determined values for all investigated parameters; however, additional research is needed to reveal exact mechanisms and implications.
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