Journal
MOLECULAR NUTRITION & FOOD RESEARCH
Volume 65, Issue 2, Pages -Publisher
WILEY
DOI: 10.1002/mnfr.202000413
Keywords
fat‐ soluble vitamins; liquid chromatography– mass spectrometry; metabolomics; phospholipids; postprandial absorption
Categories
Funding
- Foods for Health Discovery Themes Initiative SEEDS grant
- National Dairy Council
- Ohio Agricultural Research and Development Center at OSU [1007234]
- NIH [P30 CA016058, UL1TR002733]
- OSUCCC
- OSU
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Individuals with metabolic syndrome (MetS) absorb less vitamin E compared to healthy controls. This study found that MetS subjects have significantly higher absorption of vitamin A, while the absorption of vitamin D-2 is less affected by MetS status. The association between phospholipids and vitamin A suggests that enzymes may play a role in the higher bioavailability of vitamin A in MetS subjects.
Scope Persons with metabolic syndrome (MetS) absorb less vitamin E than healthy controls. It is hypothesized that absorption of fat-soluble vitamins (FSV) A and D-2 would also decrease with MetS status and that trends would be reflected in lipidomic responses between groups. Methods and Results Following soymilk consumption (501 IU vitamin A, 119 IU vitamin D-2), the triglyceride-rich lipoprotein fractions (TRL) from MetS and healthy subjects (n = 10 age- and gender-matched subjects/group) are assessed using LC-MS/MS. Absorption is calculated using area under the time-concentration curves (AUC) from samples collected at 0, 3, and 6 h post-ingestion. MetS subjects have approximate to 6.4-fold higher median vitamin A AUC (retinyl palmitate) versus healthy controls (P = 0.07). Vitamin D-2 AUC is unaffected by MetS status (P = 0.48). Untargeted LC-MS lipidomics reveals six phospholipids and one cholesterol ester with concentrations correlating (r = 0.53-0.68; P < 0.001) with vitamin A concentration. Conclusions The vitamin A-phospholipid association suggests increased hydrolysis by PLB, PLRP2, and/or PLA(2)IB may be involved in the trend in higher vitamin A bioavailability in MetS subjects. Previously observed differences in circulating levels of these vitamins are likely not due to absorption. Alternate strategies should be investigated to improve FSV status in MetS.
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