Journal
MOLECULAR BIOLOGY REPORTS
Volume 48, Issue 1, Pages 21-31Publisher
SPRINGER
DOI: 10.1007/s11033-020-06102-1
Keywords
Fluorescence in situ hybridization (FISH) 5S rDNA 45S rDNA; Chrysanthemum oligonucleotide
Categories
Funding
- National Key Research and Development Program of China [2018YFD1000401]
- Natural Science Fund of Qinghai Province, China [2018-Hz-819]
- Priority Academic Program Development of Jiangsu Higher Education Institutions
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Using rDNA as a FISH probe, researchers found that 5S rDNA sites are mainly located in subterminal chromosome regions, while the number and intensity of 45S rDNA sites differ from previously studied Chrysanthemum resources. These findings offer insight into the chromosomal structural variations in Chrysanthemum and its related species.
Fluorescence in situ hybridization (FISH) is a conventional method used to visualize the distribution of DNA elements within a genome. To examine the relationships within the Chrysanthemum genus, ribosomal DNA (rDNA), a popular cytogenetic marker, was utilized as a probe for FISH within this genus. Based on the genome data of Chrysanthemum nankingense, C. seticuspe and its allied genera in the Compositae(Asteraceae), we explored rDNA sequences to design oligonucleotide probes and perform oligonucleotide fluorescence in situ hybridization (Oligo-FISH) in eight Chrysanthemum accessions. The results showed that the majority of 5S rDNA signals were located in subterminal chromosome regions and that the number of 5S rDNA sites might be tightly associated with ploidy. For 45S rDNA sites, the number and intensity of signals differed from those of previously investigated Chrysanthemum resources. These findings may provide an optimally reliable method of examining the chromosome composition and structural variation of Chrysanthemum and its related species and allow researchers to understand the evolutionary history and phylogenetic relationships of Chrysanthemum.
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