Journal
DISEASES OF AQUATIC ORGANISMS
Volume 120, Issue 3, Pages 231-240Publisher
INTER-RESEARCH
DOI: 10.3354/dao03031
Keywords
Crassostrea gigas; Crassostrea angulata; Ostrea stentina; Herpesvirus; Microvariant; OsHV-1 mu Var; In situ hybridization
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Funding
- Operational Programme for Cross-border Cooperation between Spain and Portugal - European Regional Development Fund [0433_BONAQUA_5_E POCTEP 2007-2013]
- IFAPA - European Social Fund
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This is the first report of ostreid herpesvirus 1 microvariant (OsHV-1 mu Var) infecting natural oyster beds located in Huelva (SW Spain). The virus was detected in 3 oyster species present in the intertidal zone: Crassostrea gigas (Thunberg, 1793), C. angulata (Lamarck, 1819) and, for the first time, in Ostrea stentina Payraudeau, 1826. Oysters were identified by a specific polymerase chain reaction (PCR) and posterior restriction fragment length polymorphism (RFLP) analysis based on cytochrome oxidase I (COI) mitochondrial DNA. Results confirmed that C. angulata still remains the dominant oyster population in SW Spain despite the introduction of C. gigas for cultivation in the late 1970s, and its subsequent naturalization. C. angulata shows a higher haplotype diversity than C. gigas. OsHV-1 virus was detected by PCR with C2/C6 pair primers. Posterior RFLP analyses with the restriction enzyme MfeI were done in order to reveal the OsHV-1 mu Var. Detections were confirmed by DNA sequencing, and infections were evidenced by in situ hybridization in C. gigas, C. angulata and O. stentina samples. The prevalence was similar among the 3 oyster species but varied between sampling locations, being higher in areas with greater harvesting activities. OsHV-1 mu Var accounted for 93% of all OsHV-1 detected.
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