Journal
MICROCHIMICA ACTA
Volume 188, Issue 2, Pages -Publisher
SPRINGER WIEN
DOI: 10.1007/s00604-020-04652-6
Keywords
Phosphopeptides; Enrichment; Metal ion affinity chromatography (IMAC); MALDI-TOF MS
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Funding
- Zhejiang Natural Science Foundation [LQ19C050002]
- State Key Laboratory of Analytical Chemistry for Life Science [SKLACLS1901]
- National Natural Science Foundation of China [511903050]
- K. C. Wong Magna Fund in Ningbo University
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The Fe3O4@mSiO(2)@APA@Ti4+/Nb5+ material has shown promising potential in effectively enriching global phosphopeptides, with high selectivity, low detection limit, good reproducibility, and excellent loading capacity.
A strategy for effectively enriching global phosphopeptides was successfully developed by using ammonia methyl phosphate (APA) as a novel chelating ligand and Ti4+ and Nb5+ as double functional ions (referred to as Fe3O4@mSiO(2)@APA@Ti4+/Nb5+). With the advantage of large specific surface area (151.1 m(2)/g), preeminent immobilized ability for metal ions (about 8% of total atoms), and unbiased enrichment towards phosphopeptides, Fe3O4@mSiO(2)@APA@Ti4+/Nb5+ displays high selectivity (maximum mass ratio beta -casein to BSA is 1:1500), low limit of detection (LOD, as low as 0.05 fmol), good relative standard deviation (RSD, lower than 7%), recovery rate of 87% (O-18 isotope labeling method), outstanding phosphopeptide loading capacity (330 mu g/mg), and at least five times re-use abilities. In the examination of the actual sample, 24 phosphopeptides were successfully detected in saliva and 4 phosphopeptides were also selectively extracted from human serum. All experiments have shown that Fe3O4@mSiO(2)@APA@Ti4+/Nb5+ exhibits exciting potential in view of the challenge of low abundance of phosphopeptides.
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