Enantioselective electrochemical sensor of tyrosine isomers based on macroporous carbon embedded with sulfato-β-Cyclodextrin

This work reports an enantioselective electrochemical sensor toward tyrosine (Tyr) enantiomers based on macroporous carbon embedded with sulfato-beta-cyclodextrin (MPC-SCD) hybrid. First, MPC-SCD hybrid was synthesized via adsorption of sulfato-beta-cyclodextrin (SCD) by macroporous carbon (MPC) in aqueous solution, and characterized using transmission electron microscopy (TEM), scanning electron microscopy (SEM), thermogravimetric analysis (TGA), and Fourier transform infrared spectroscopy (FTIR). Subsequently, an enantioselective electrochemical sensor was fabricated by modifying glassy carbon electrode (GCE) with MPC-SCD hybrid, and its electrochemical performances were characterized by electrochemical impedance spectroscopy (EIS) and cyclic voltammograms (CV). Furthermore, the electrochemical sensor was utilized for chiral analysis toward Tyr enantiomers by differential pulse voltammograms (DPV). The peak currents of Tyr enantiomers increased linearly with increasing concentrations over the range of 1-500 mu M. In addition, the sensitivity were calculated as 84.59 and 73.02 mu A/mM for D-Tyr and L-Tyr, respectively, and the detection limits were 0.20 and 0.26 mu M. Furthermore, multiple linear regression (MLR) chemometrics modeling method was used for simultaneously analysis of Tyr enantiomers. The presented sensor could provide a promising protocol for designing novel electrochemical platforms in the field of chiral analysis.