Journal
MICROBIAL PATHOGENESIS
Volume 149, Issue -, Pages -Publisher
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.micpath.2020.104551
Keywords
Carp edema virus; Koi; Histopathology; qPCR
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Funding
- Natural Science Foundation of Tianjin city [18JCYBJC29900]
- Open Fund of Tianjin Key Lab of Aquatic Ecology and Aquaculture [TJAE201803]
- Key Scientific Research Project Universities and Colleges in Tianjin [2019ZD14]
- Innovation Team of Tianjin Fisheries Research System [ITTFRS2017009]
- Tianjin agricultural university Key Laboratory of Aqua-ecology platform project [J01009030638]
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Carp edema virus disease (CEVD) has resulted in great economic losses in koi (Cyprinus carpio koi) and common carp (Cyprinus carpio carpio) populations in the world. In this study, the diseased koi were diagnosed as CEV infection based on 5' untranslated region (5'UTR) and 4a protein genes by the conventional PCR, nested PCR and quantitative PCR (qPCR) analyses. Phylogenetic tree analysis showed that the TJ201708 strain was classified into the genogroup IIa. Furthermore, qPCR of 5'UTR gene revealed that the lowest detection limit was 4.0 fg/mu L. The pathogenicity of CEV for koi was demonstrated in the infection experiments. Histopathological examination revealed the petechial hemorrhages of liver and spleen, vacuolization of lamina propria of intestine and swelling and necrosis of respiratory epithelial cells of gills. To our knowledge, this is the first report the qPCR of 5'UTR gene in the detection of carp edema virus.
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