Greener synthesis of cerium oxide nanoemulsion using pollen grains of Brassica napus and evaluation of its antitumour and cytotoxicity properties.

In this project, the CeO2NPs were synthesized using pollen grains of Brassica napus as a capping agent and reducing agent. The nanoceria were characterized by FTIR, FESEM, TEM, PXRD, and DLS. To increase the bioavailability, the nanoemulsion of cerium nanoparticle was prepared. The TEM images have revealed particles with spherical morphology and mean particle sizes of ca. 23.2 +/- 4.0 nm. The prepared sample after calcination at 500 degrees C showed the characteristic peaks of nanoceria with a good crystallinity. Chorioallantoic membrane (CAM) test results showed by increasing the concentration of biosynthesized CeO2NPs, the size and number of blood vessels were reduced. Also, the cytotoxicity test using MTT showed little toxicity of the biosynthetic CeO2NPs on the human foreskin fibroblast cell, whereas synthetic CeO2NPs destroyed ovarian cancer cells (A2780) which depends on the time and concentration of the NPs. The results of this research show excessive promise for drug treatments and pharmacological technology.

Automated summary

CeO2NPs were synthesized using pollen grains of Brassica napus as a capping and reducing agent. Various characterizations were performed on the nanoceria. The prepared sample showed good crystallinity after calcination. The biosynthesized CeO2NPs showed potential effects on blood vessels and cells, indicating promising applications.