Journal
MATERIALS SCIENCE AND ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS
Volume 120, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.msec.2020.111787
Keywords
Electrospinning; Fiber alignment; Fiber diameter; Cellular behaviors; Osteogenesis
Categories
Funding
- National Natural Science Foundation of China [81873715, 82072396]
- Shanghai Collaborative Innovation Center for Translational Medicine [TM202010]
- Science and Technology Commission of Shanghai Municipality [201409006200, 18441902100]
- Program of Shanghai Academic/Technology Research Leader [19XD1434500]
- Two-Hundred Talent [20191819]
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Electrospun fiber scaffolds, mimicking the bone extracellular matrix, have become important biomaterials in bone tissue engineering. The topographic cues of aligned nanofibers were found to significantly influence BMSCs cellular responses, including morphology, migration, proliferation, and osteogenesis. Aligned nanofiber scaffolds demonstrated higher osteogenic induction proficiency compared to aligned microfibers and random fiber substrates.
Electrospun fiber scaffolds, due to their mimicry of bone extracellular matrix (ECM), have become an important biomaterial widely applied in bone tissue engineering in recent years. While topographic cues of electrospun membranes such as alignment and diameter played vital roles in determining cellular behaviors. Yet few researches about the effects of these two significant parameters on osteogenesis have been reported. Thus, the present work explored the influence of aligned and random poly (L-lactic acid) (PLLA) fiber matrices with diameters of nanoscale (0.6 mu m) and microscale (1.2 mu m), respectively, on cellular responses of bone marrow mesenchymal stem cells (BMSCs), such as cell adhesion, migration, proliferation and osteogenesis. Our results revealed that aligned nanofibers (AN) could affect cell morphology and promote the migration of BMSCs after 24 h of cell culturing. Besides, AN group was observed to possess excellent biocompatibility and have significantly improved cell growth comparing with random nanofibers. More importantly, in vitro osteogenesis researches including ALP and Alizarin Red S staining, qRT-PCR and immunofluorescence staining demonstrated that BMSCs culturing on AN group exhibited higher osteogenic induction proficiency than that on aligned microfibers (AM) and random fiber substrates (RN and RM). Accordingly, aligned nanofiber scaffolds have greater application potential in bone tissue engineering.
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