Journal
LIFE SCIENCES
Volume 265, Issue -, Pages -Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.lfs.2020.118758
Keywords
IGFBP-3; VCAM-1; Migration; Osteosarcoma; Metastasis
Funding
- Ministry of Science and Technology of Taiwan [MOST 107-2320-B-039-019-MY3, MOST-108-2314-B-039-034-MY3, 106-2314-B-341-001-MY3]
- China Medical University Hospital [DMR-110-158]
- Taichung Hospital, Ministry of Health and Welfare [10519, 10623, 10913]
- Shin Kong Wu Ho-Su Memorial Hospital [107-SKH-FJU-07]
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IGFBP-3 levels were found to be significantly higher in osteosarcoma tissue compared to normal tissue, and IGFBP-3 promoted cell migration in osteosarcoma cells by upregulating VCAM-1 expression through PI3K/Akt and AP-1 signaling pathways, suggesting IGFBP-3 as a potential therapeutic target in metastatic osteosarcoma.
Aims: Insulin-like growth factor (IGF) signaling has been documented in several human malignancies and is thought to contribute to cellular differentiation and migration, as well as malignant progression. A major binding molecule of IGF, IGF-binding protein 3 (IGFBP-3), regulates multiple IGF effects. Here, we focused on the effect of IGFBP-3 in the motility of osteosarcoma cells and examined signaling regulation. Materials and methods: Using a human osteosarcoma tissue array, immunohistochemical staining determined levels of IGFBP-3 expression in osteosarcoma tissue and in normal tissue. The wound healing migration assay, Transwell migration assay, luciferase reporter assay, immunofluorescence staining, Western blot and real-time quantitative PCR were performed to examine whether IGFBP-3 facilitates VCAM-1-dependent migration of osteosarcoma cells. Key findings: In this study, we found significantly higher IGFBP-3 levels in osteosarcoma tissue compared with normal healthy tissue. IGFBP-3 treatment of two human osteosarcoma cell lines promoted cell migration and upregulated levels of VCAM-1 expression via PI3K/Akt and AP-1 signaling. Significance: IGFBP-3 appears to be a novel therapeutic target in metastatic osteosarcoma.
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