Journal
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
Volume 32, Issue 2, Pages 611-614Publisher
AMER CHEMICAL SOC
DOI: 10.1021/jasms.0c00336
Keywords
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Funding
- NSF [CHE-1411376]
- NSF SBIR Phase I [1913787]
- Faculty of Mathematics and Natural Sciences, University of Oslo, Oslo, Norway
- Div Of Industrial Innovation & Partnersh
- Directorate For Engineering [1913787] Funding Source: National Science Foundation
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This study evaluated the use of vacuum matrix-assisted ionization mass spectrometry for identification and determination of tryptic peptides from ProGRP protein. The vacuum ionization conditions lowered the detection limits and enabled detection of more digested protein peptides. Coupling with high resolution MS, vMAI showed advantages in determining MS/MS fragmentation conditions and differentiation of intact protein isoforms.
This initial study evaluates vacuum matrix-assisted ionization (vMAI) mass spectrometry (MS) for identification and determination of tryptic peptides from the biomarker protein progastrin releasing peptide (ProGRP). Similar peptides and charge states were observed as in liquid chromatography (LC) electrospray ionization (ESI) MS. The prolonged ion duration in vMAI with similar charge states as in ESI was advantageous for determining the MS/MS fragmentation conditions compared to MAI. It is assumed that the vacuum ionization conditions lower the detection limits of the experiment. This may be the reason vMAI combined with high resolution MS enabled detection of tryptic peptides from more digested proteins than MAI selected reaction monitoring MS. Additionally, MAI ion mobility spectrometry MS (MAI-IMS-MS) was evaluated for differentiation of intact protein isoforms, successfully enabling differentiation of the isoforms by drift time selection. Examples are both shown for model proteins bovine serum albumin, cytochrome C, and lysozyme and the clinically relevant small cell lung cancer protein biomarker ProGRP, which exists in three isoforms. Coupling with the vacuum ionization conditions using a dedicated vacuum-probe source MAI enables information to be extracted readily as with conventional approaches, just faster.
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