Journal
JOURNAL OF SEPARATION SCIENCE
Volume 44, Issue 5, Pages -Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/jssc.202001087
Keywords
origin tracing; principal component analysis; quality assessment; traditional Chinese medicine; triple quadrupole tandem mass spectrometry
Categories
Funding
- National Natural Science Foundation of China [81973471, 82003937]
- Double First-ClassUniversity Project [CPU2018GF06, CPU2018GY32]
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Through various chromatographic analysis methods and mass spectrometry techniques, this study successfully detected and classified the ingredients of Angelicae Sinensis Radix from six different geographical zones, providing a convenient and reliable approach for origin tracing and quality control of Angelicae Sinensis Radix.
Angelicae Sinensis Radix is a world-renowned herbal medicine originating in China. Owing to many environmental and geographical factors, Angelicae Sinensis Radix from various origins may have a difference in the content of ingredients, which made the confusion in the clinical practice and market. Herein, a binary chromatographic fingerprinting analysis method is developed via hydrophilic interaction chromatography and reversed-phase liquid chromatography to obtain more chemical information. Following that, an ultra-performance liquid chromatography with a triple quadrupole mass spectrometry method is furnished to simultaneously detect 17 ingredients of Angelicae Sinensis Radix gathered from six geographic zones in China. Eventually, the principal component analysis is successfully carried out to classify and differentiate the Angelicae Sinensis Radix from different origins, meanwhile the quantitative volcano plots was used to observe the changes of ingredient trends vividly. Accordingly, the proposed binary chromatography and triple quadrupole tandem mass spectrometry coupled with multivariate statistical analysis can be utilized as a facile and reliable method for origin tracing and quality control of Angelicae Sinensis Radix.
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