Journal
JOURNAL OF RECEPTORS AND SIGNAL TRANSDUCTION
Volume 42, Issue 1, Pages 23-33Publisher
TAYLOR & FRANCIS LTD
DOI: 10.1080/10799893.2020.1839764
Keywords
Pulsatilla saponin E; non-small cell lung cancer; flotillin-2; Akt; FASN pathway
Categories
Funding
- Zhejiang Traditional Chinese Medicine Research Fund Project [2019ZA092]
- Key Project of Hangzhou Health Science and Technology Plan [2018Z04]
Ask authors/readers for more resources
This study revealed that pulsatilla saponin E can suppress migration and invasion while promoting apoptosis in non-small cell lung cancer cells through negatively regulating the Akt/FASN signaling pathway. These findings could be valuable in the development of novel therapeutic drugs for NSCLC treatment.
Purpose Pulsatilla saponins from pulsatilla chinensis (Bunge) Regel have potential anti-tumor activities to certain human cancers. However, the roles of pulsatilla saponin E separated from pulsatilla saponins in non-small cell lung cancer (NSCLC) have not been reported. Materials and methods After treating NSCLC cells by pulsatilla saponin E at different concentrations, cell viability was measured by MTT and CCK-8 assays, and cell migration, invasion and apoptosis were detected by scratch wound-healing, transwell and flow cytometry assays. The contents of free cholesterol (FC) and total cholesterol (TC) were measured by high performance liquid chromatography (HPLC). The expression levels of flotillin-1, flotillin-2, Akt, fatty acid synthase (FASN) were detected by qRT-PCR and Western blot assays. Results Pulsatilla saponin E suppressed viability, migration, invasion and promoted apoptosis of NSCLC cells followed by regulation of apoptosis-related proteins, reduced contents of FC and TC, and the expression levels of flotillin-1, flotillin-2, Akt, and FASN in a concentration-dependent manner. However, the inhibitory effects of pulsatilla saponin E on viability, migration, invasion of A549 cells and the expression levels of flotillin-1, flotillin-2, Akt, and FASN were reversed by flotillin-2 overexpression. Conclusions Our study revealed that pulsatilla saponin E suppressed migration, invasion and promoted apoptosis of NSCLC cells through negatively regulating Akt/FASN signaling pathway via the inhibition of flotillin-2 in lipid raft (LR). The current findings could be explored for developing a novel therapeutic drug for NSCLC treatment.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available