Journal
JOURNAL OF RADIOANALYTICAL AND NUCLEAR CHEMISTRY
Volume 327, Issue 2, Pages 713-719Publisher
SPRINGER
DOI: 10.1007/s10967-020-07549-z
Keywords
Bee pollen; Gamma radiation; EPR; Free radicals; Antioxidant activity; Total polyphenol content
Funding
- Bulgarian National Science Fund at the Bulgarian Ministry of Education [KP-06-N 39/12]
- Bulgarian Ministry of Education and Science [D01-284/17.12.2019]
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The study used EPR spectroscopy to evaluate the gamma-induced free radicals and antiradical activity of bee pollen. It was found that gamma irradiation affects the antioxidant activity of pollen samples and there is a difference in radical scavenging activities between different batches of pollen before and after irradiation. Additionally, the results from EPR spectroscopy correspond well with the determined contents of polyphenol compounds.
Gamma-induced free radicals and antiradical activity of bee pollen were evaluated by means of Electron Paramagnetic Resonance (EPR) spectroscopy. Total phenolic and flavonoid contents were spectrophotometrically determined. Two bee pollen samples of consecutive crops were exposed to gamma-irradiation. The O-centered free radicals were induced by gamma rays. Their signal increased upon increasing the dose used. The fading kinetics of free radicals was different for the two samples and they were stable for 5 months. The irradiation effect on radical scavenging activity of pollen extracts was investigated by using the stable free radical 1,1-diphenyl-2-picrylhydrazyl (DPPH) and the antioxidant activity was represented in Trolox Equivalents. It was found out that the gamma irradiation affects the antioxidant activity of pollen samples. There is a difference between the radical scavenging activities of the two samples before and after irradiation. The EPR results are in good correspondence with the determined contents of polyphenol compounds.
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