Journal
JOURNAL OF PLANT PATHOLOGY
Volume 103, Issue 2, Pages 629-634Publisher
SPRINGER
DOI: 10.1007/s42161-021-00764-x
Keywords
Radish (raphanus sativus); Leaf curl disease; Papaya leaf curl virus (PaLCuV); Papaya leaf curl betasatellite (PaLCuB)
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Funding
- CSIR, New Delhi [BSC 0117]
- Department of Science and Technology (DST-INSPIRE-Faculty scheme) [IFA-17LSPA-100]
- Indian Councel of Medical Research (ICMR)-SRF [2020-7629]
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The study identified severe leaf curl symptoms on radish plants in cultivated fields in Lucknow, caused by a begomovirus and its associated betasatellite transmitted by whiteflies. Molecular analysis showed close phylogenetic relationships with papaya leaf curl virus (PaLCuV) and its betasatellite (PaLCuB).
Radish (Raphanus sativus L.) plants of the cultivar Pusa Reshmi exhibiting severe leaf curl symptoms were observed in the cultivated fields at Lucknow with significant disease incidence. The virus was whitefly transmittable and induced leaf curl symptoms by 24 days post inoculation. PCR with two sets of degenerate primers confirmed the presence of a begomovirus and its associated betasatellite. Further, the full-length viral genome was amplified by RCA and two clones of similar to 2.7 kb were sequenced (KY026598 and KY026597) which shared the highest (95.25%) sequence identity and close phylogenetic relationships with papaya leaf curl virus (PaLCuV), therefore, identified as isolates of PaLCuV. The associated betasatellite was identified as papaya leaf curl betasatellite (PaLCuB) based on its highest sequence identity (98.32%) and close relationship with PaLCuB.
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