A cell-compatible red light-emitting multianalyte chemosensor via three birds, one stone strategy

The design and synthesis of red light-emitting multianalyte chemosensors have always been a challenging task because of its specific requirement of coordination pocket and selective fluorescence mechanism. Herein, we develop a chemosensor via three birds, one stone strategy in which we can detect three metal ions with one ligand. A highly sensitive new azo functionalized rhodamine based luminescent sensor is synthesized for se-lective fluorogenic recognition of Al3+, Cr3+, and chromogenic recognition of Cu2+ in ethanol : H2O medium in the red light-emitting zone. Among the guest metals, Cu2+ efficiently quenches the emission whereas Al3+ and Cr3+ induce increased luminescent 4.76 fold for Al3+ and 2.47 fold for Cr3+ through chelation-enhanced fluorescence (CHEF) and photo-induced electron transfer (PET) regulated mechanism with the formation of 1:1 complex. The restricted imine isomerization through complex formation inhibits ongoing PET process with the instantaneous onset of CHEF. The mechanism is in good consonance with NMR (1H & 13C), FT-IR, elemental analysis, DFT, TCSPC, and pH-dependent studies. Micromolar range detection of 1.1 mu M, 1.3 mu M, and 1.5 mu M for Cu2+, Al3+, and Cr3+ respectively, easy penetration into HLCs cells and higher imaging resolution increase its potentiality to assess Al3+ and Cr3+ in vitro. Moreover, paper strip application increases its viability as an onsite naked-eye portable solid probe.

Automated summary

The study successfully synthesized a new luminescent sensor that can detect three metal ions with high sensitivity and selectivity. The different effects of different metal ions allowed for efficient recognition and detection of Al3+, Cr3+, and Cu2+.