Journal
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
Volume 192, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.jpba.2020.113674
Keywords
Pueraria candollei; Methylisomiroestrol; Monoclonal antibody; ELISA; HPLC
Categories
Funding
- Royal Golden Jubilee Ph.D. program, National Research Council of Thailand, Thailand Science Research and Innovation [PHD/0071/2560]
- International Research Network for Functional Food Discovery AMP
- Development (FFDD) of Thailand Science Research and Innovation [IRN61W0005]
- Khon Kaen University
- Ministry of Health, Labour and Welfare, Japan [19KA3003]
Ask authors/readers for more resources
The developed ELISA method is effective in detecting trace amounts of methylisomiroestrol in Pueraria candollei, which is crucial for standardization of the products and improving the quality of plant-derived products.
Pueraria candollei is a phytoestrogen-rich herb used to treat estrogen deficiency disorders; however, quality control of P. candollei-related health products is required for consistency of clinical outcomes. Estrogenically active (+)-7-0-methylisomiroestrol could be a potential chemical marker that facilitates the prediction of the overall estrogenic activity of P. candollei. The analytical performance of ELISA using newly produced monoclonal antibodies against methylisomiroestrol was compared with HPLC analysis. The developed indirect competitive ELISA (icELISA) was highly sensitive to methylisomiroestrol for detection, with an LOQof 2.9 ng/mL, whereas the LOQ was 1.15 mu g/mL by HPLC. The results from method validation indicated acceptable precision (1.71-6.37 % and 0.13-2.40 %) and accuracy (99.23-102.54 % and 96.84-101.88 %) of the methylisomiroestrol analysis using icELISA and HPLC. These methods were effectively applied for the determination of the methylisomiroestrol content in P. candollei samples. Apart from the plant tubers, the stem was observed as a source of methylisomiroestrol. The developed ELISA was more effective than HPLC in detecting a small quantity of methylisomiroestrol in the plant samples [0.23 x 10(-3) % (w/w) to 0.628 x 10(-3) % (w/w) dry weight]. Therefore, the ELISA could be a useful tool for the standardization of P. candollei, which is the crucial step to improve the quality of plant-derived products. (C) 2020 Elsevier B.V. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available